Translation in Xenopus oocytes of mRNAs transcribed in vitro E.E.
Isolation of total and polysomal RNA from plant tissues Sacco de Vries, Harry Hoge & Ton Bisseling 7. Subcellular targeting of proteins in vivo and in vitro P.H. Analysis of gene expression in transgenic plants Ferenc Nagy, Steve A. Assays for studying DNA methylation Aharon Razin 4. Assays for studying chromatin structure Anna-Lisa Paul & Robert J. Use of reporter genes to study gene expression in plant cells Luis Herrera-Estrella, Teemu H. SECTION B: Expression of Genes in Plants 1. Transformation of plant pathogenic fungi Robert C. Selectable and screen able markers Arlette Reynaerts, Marc de Block, Jean-Pierre Hernalsteens & Marc van Montagu 10. Procedures for constructing genomic clone banks Jerry L. Procedures for contructing ds-cDNA clone banks Jerry L. Extraction of DNA from plant tissues Scott O. Horsch, Joyce Fry, Nancy Hoffmann, Jeanne Neiderrneyer, Stephen G. Agrobacterium molecular genetics Paul J.J. Use of cointegrating Ti plasmid vectors Stephen G. Direct DNA transfer to protoplasts with and without electroporation Michael W. V Contents SECTION A: Introduction of DNA into Plant Cells 1. without the prior written permission of the publishers. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, mechanical, photocopying, recording, or otherwise. Box 322, 3300 AH Dordrecht, The NetherlandsĬopyright © 1989 by Kluwer Academic Publishers, Dordrecht.Īll right reserved. Box 358, Accord Station, Hingham, MA 02018-0358, USA for the UK and Ireland: Kluwer Academic Publishers, MTP Press Limited, Falcon House, Queen Square, Lancaster LAI lRN, UK for all other countries: Kluwer Academic Publishers Group, Distribution Center, P.O. Kluwer Academic Publishers Dordrecht / Boston / Londonįor the United States and Canada: Kluwer Academic Publishers, P.O. VERMA Ohio State University, Columbus Ohio, USA SCHILPEROORT Leiden State University, Leiden The Netherlands DESH PAL S. GELVIN Purdue University, West Lafayette Indiana, USA ROBBERT A.
PLANT MOLECULAR BIOLOGY MANUAL edited by: A list of the various contributors to this manual, including their addresses, is included. Those people desiring such material should contact the relevant authors directly. The editors recognize that many of the techniques described in this manual depend upon specialized plant genetic material, microbial strains, or recombinant plasmids. Additional chapters that can replace or be added to this first edition will be published periodically. Recognizing the rapidly changing nature of plant molecular biology technology, the editors have designed a laboratory manual that is both easy to use in the laboratory and which will be updated as the techniques change and new technologies are devised. The manual is designed both for advanced college level laboratory courses and as a 'bench guide' for use in the scientific laboratory. In 1986, some of us felt that many of these techniques had become routine enough to warrant the publication of a laboratory manual. Along with this new technology has come an almost bewildering array of rapidly changing techniques, often making it difficult for the novice to select and perform the technique most appropriate for answering a given biological question. Such formerly esoteric techniques as gene transfer and plant regeneration are now routinely performed, making the dissection of regulatory elements of genes a common practice in many laboratories.
During the past ten years, great advances have been made in the area of plant molecular biology.